Fig. 4

A diagram of the cell showing processes associated with key mutations. A cartoon diagram of key mutations in potential causal genetic regions identified in the evolved strains. A total of eight mutations are represented amongst different genetic regions. A Δ120 bp deletion was found in the non-coding region of mdtJ, near the promoter, and its neighboring gene tqsA. Two other structural changes were found in the tqsA gene—one was an intragenic in-frame Δ12 bp deletion, the other was a Δ3035 bp deletion which included a major section of tqsA and the pntB and pntA genes located next to tqsA on the chromosome. Finally, five structural changes were identified in yhdP gene. These were two out-of-frame short deletions (Δ2 bp and Δ4 bp), two intergenic IS mobile element insertions, and a short 7 bp insertion/duplication. These mutations indicate a probable loss-of-function of yhdP