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Fig. 1 | Microbial Cell Factories

Fig. 1

From: LesR is a novel upstream regulator that controls downstream Clp expression to modulate antibiotic HSAF biosynthesis and cell aggregation in Lysobacter enzymogenes OH11

Fig. 1

Introduction of clp into the overexpression strain of lesR. a Plasmid construction leading to overexpression of clp under the control of its native promoter in the background of lesR overexpression. b RT-PCR assay of lesR or clp in the respective transformed L. enzymogenes strain. The gene 16S rRNA was used as an internal control as described previously [14]. OH11(pBBR), the wild-type strain with an empty vector; OH11(lesR), the lesR overexpression strain; OH11(clp), the clp overexpression strain; and OH11(lesR-clp), the introduction of clp into OH11(lesR)

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