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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Prokaryotic expression and characterization of the heterodimeric construction of ZnT8 and its application for autoantibodies detection in diabetes mellitus

Fig. 6

Immunochemical characterization of TrxZnT8-biotin. a Dose–response curves for a pool of 5 ZnT8A RBA positive type 1 diabetic patient sera for different concentrations of TrxZnT8 from IB (open circle, solid line) and TrxZnT8-biotin (closed circle, dotted line). b Inhibition capacity of TrxZnT8-biotin assessed in 32 ZnT8A RBA positive type 1 diabetic patient sera in the absence (B) or presence (C) of TrxZnT8-biotin. Fourty control sera were used in order to set a cut-off value (A). The binding was expressed as SDs, and the dotted line represents the cut-off value

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