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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Prokaryotic expression and characterization of the heterodimeric construction of ZnT8 and its application for autoantibodies detection in diabetes mellitus

Fig. 4

Purification of TrxZnT8 from IB by affinity chromatography. Analysis of TrxZnT8 fractions at different stages of purification by a SDS-PAGE (12.1% T, 6.0% C, 1 mm, under reducing conditions, stained with Coomassie Brillant Blue R-250) and b WB revealed with a rabbit polyclonal serum to thioredoxin as primary antibody. Lane 1 corresponds to total cell lysate from transformed E. coli strain GI724, lane 2 TrxZnT8 from IB solubilized with 8 M urea, lane 3 TrxZnT8 in oxidative refolding buffer, lane 4 unbound material, and lane 5 purified TrxZnT8 from IB. Arrows indicates the electrophoretic mobility of TrxZnT8

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Microbial Cell Factories

ISSN: 1475-2859

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