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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Prokaryotic expression and characterization of the heterodimeric construction of ZnT8 and its application for autoantibodies detection in diabetes mellitus

Fig. 3

Immunochemical characterization of TrxZnT8 from ISF. a Dose–response curves for 5 ZnT8A RBA positive type 1 diabetic patient sera incubated with different concentrations of TrxZnT8. Each curve includes best-fit values from the log (inhibitor) vs. response–variable slope (four parameters) equation: correlation coefficient data (R2), IC50 values and approximation to the affinity constant of ZnT8A (Ka) calculated as the inverse of IC50. b Inhibition capacity of TrxZnT8 assessed in 29 ZnT8A RBA positive type 1 diabetic patient sera in the absence (B) or presence (C) of TrxZnT8. Twenty control sera were used in order to set a cut-off value (A). The binding was expressed as SDs, and the dotted line represents the cut-off value

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