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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Prokaryotic expression and characterization of the heterodimeric construction of ZnT8 and its application for autoantibodies detection in diabetes mellitus

Fig. 2

Biochemical characterization of TrxZnT8. a Mass spectrometry analysis of recombinant TrxZnT8. The box indicates the experimental peak corresponding to TrxZnT8. b TrxZnT8 scheme (above) and sequence (below). In the scheme, N-terminal Trx fraction, the linker peptide and the ZnT8 fragment in the C-terminal of the construction are detailed, and the corresponding peptides fragments identified by Orbitrap Mass Spectrometry are highlighted in grey in the diagram. Also, the TrxZnT8 sequence is represented: underlined and highlighted in bold are those fragments identified by Orbitrap Mass Spectrometry considering high confidence peptides

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