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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Prokaryotic expression and characterization of the heterodimeric construction of ZnT8 and its application for autoantibodies detection in diabetes mellitus

Fig. 1

Purification of TrxZnT8 from ISF by affinity chromatography. Analysis of TrxZnT8 fractions at different stages of purification by a SDS-PAGE (12.1% T, 6.0% C, 1 mm, under reducing conditions, stained with Coomassie Brilliant Blue R-250) and b WB revealed with a rabbit polyclonal serum to thioredoxin as primary antibody. In a and b, lane 1 corresponds to total ISF from transformed E. coli strain GI698, lane 2: unbound material, lane 3 and 4: washes with increasing 2ME concentrations (1 and 5 mM), lanes 5 and 6: 100 mM consecutive eluates of purified TrxZnT8. Arrows indicate the electrophoretic mobility of TrxZnT8

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Microbial Cell Factories

ISSN: 1475-2859

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