Table 1 CRISPR/Cas9-mediated gene deletion and insertion in C. glutamicum wild-type strains
From: Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum
Strain | Plasmids used | Deleted fragment size (bp) | Inserted fragment size (bp) | Homologous arm size (bp) | Efficiency |
|---|---|---|---|---|---|
ATCC 13869 | pCas9 and pgRNA3 | 664 | 0 | ~Â 1000 | 30.8% (4/13); 35.7% (5/14); 33.3% (2/6) |
pCas9 and pgRNA4 | 664 | 781 | ~Â 1000 | 28.6% (4/14) | |
ATCC 13032 | pCas9 and pgRNA3 | 664 | 0 | ~Â 1000 | 60.0% (9/15) |
pCas9 and pgRNA8 | 664 | 0 | ~Â 500 | 50.0% (2/4) | |
pCas9 and pgRNA9 | 8083 | 0 | ~Â 1000 | 40.0% (2/5) | |
pCas9 and pgRNA4 | 664 | 781 | ~Â 1000 | 62.5% (5/8) | |
pCas9 and pgRNA10 | 0 | 3626 | ~Â 1000 | 50.0% (4/8) |