Table 1 The PAM site and the sgRNA sequence used in this study
From: Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system
Gene | PAM sequence | PAM site and gene length | Target strand | GC (%) |
|---|---|---|---|---|
porB | GGAGGATAGGTTTGCGAAGTCGG | 83/381 | NT | 50 |
mepA | GGCACCT TCACCTCAGGATTCGG | 214/576 | T | 55 |
clpX | GGCTGAAATCTCCGACGGCTTGG | 194/1281 | NT | 60 |
Ncgl0911 | GGTAACTGGGCTGGCCAAAAGGG | 62/1260 | NT | 55 |
mepA1 | GGTGGCGGTAGCGGTTGCGGTGG | 62/576 | NT | 75 |
mepA2 | GGATGCTGGTGCCATGGTGGCGG | 77/576 | NT | 65 |
mepA3 | GGAGGAAAGGCCTGCGTAGTCGG | 108/576 | NT | 60 |
mepA4 | GGCGGCTGGTGCGACGGCGGTGG | 173/576 | NT | 85 |
mepA5 | GGCCAGGAAATCGCAGGAATGGG | 445/576 | T | 55 |
mepA6 | GGCAGCCAAGGATTCTCCACCGG | 467/576 | T | 60 |