Fig. 7
From: Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system
GFP expression in the porB, mepA, clpX and Ncgl0911-deleted strains. CK is a negative control of the wild-type strain containing pXMJ19 without the gfp gene. WT-GFP is a positive control of the wild-type strain containing pXMJ19-EGFP; MmepA-GFP is the mepA-deleted mutant with plasmid pXMJ19-EGFP; MmepA-mepA-GFP is the mepA-deleted mutant complemented by mepA gene and containing pXMJ19-EGFP; MporB-GFP is the porB-deleted mutant with plasmid pXMJ19-EGFP; MporB-porB-GFP is the porB-deleted mutant complemented by porB gene and containing pXMJ19-EGFP. a Fluorescence intensities normalized against culture OD600 were used to indicate the expression of GFP. b The expression of GFP was determined by fluorescence microscopy with an exposure time of 1Â s. The mepA and porB mutants showed GFP expression enhanced by 55.2 and 62.4%, respectively, relative to the wild type