Fig. 4
From: Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system
Genome editing mediated by CRISPR/Cas9 in C. glutamicum ATCC 13032. a Schematic depicting editing procedures, the PAM site and gene length. b Disruption of the mepA gene mediated by the CRISPR/Cas9 system in C. glutamicum ATCC 13032. The editing efficiency was 2/15. The lane ‘ck’ is the PCR product from the wild-type strain. c Disruption of the clpX gene mediated by the CRISPR/Cas9 system in C. glutamicum ATCC 13032. The editing efficiency was 5/16. d Disruption of the Ncgl0911 gene mediated by the CRISPR/Cas9 system in C. glutamicum ATCC 13032. The editing efficiency was 4/15