Fig. 1
From: Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system
Design of the CRISPR/CAS9 system for gene deletion in C. glutamicum. a Strategy for construction of pFSC. Cas9 is controlled by the IPTG-inducible Ptac promoter, the SD sequence is designed to enhance the expression of Cas9; b strategy for construction of pFST. The sgRNA cassette is under the control of the IPTG-inducible Ptrc promoter, the 20 nt target sequence is shown in gold, the backbone is a temperature sensitive repA replicon, the HDarm is ligated into pFST at the BglII site; c strategy for construction of the sgRNA. The red N20 is the 20 nt target sequence, and the underlined sequences are the sgRNA scaffold. The EcoRI and XbaI sites are used to assemble the sgRNA into pFST