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Table 2 Nucleotide composition and codon adaptation index of the expressed and non-expressed genes

From: Effects of mRNA secondary structure on the expression of HEV ORF2 proteins in Escherichia coli

 

Parameters

Expressed genes

Non-expressed genes

Average

SD

Average

SD

Nucleotide composition

A

18.68

0.46

18.83

0.53

C

26.75

0.29

26.82

1.05

T

32.32

0.96

32.83

1.53

G

22.25

0.92

21.52

0.52

G + C

49

0.72

48.34

1.17

A1a

22.63

1.8

23.28

1.24

C1

23.98

1.03

24.82

2.34

T1

21.46

1.23

29.07

1.81

G1

31.93

1.45

22.84

2.13

G1 + C1

55.91

1.95

47.65

1.44

A2b

26.79

2.12

17.07

3.83

C2

34.17

2.35

32.13

3.29

T2

23.75

0.52

30.95

1.1

G2

15.3

2.7

19.85

2.73

G2 + C2

49.46

2.11

51.98

1.79

A3c

6.63

0.34

16.13

1.56

C3

22.1

0.73

23.52

2.25

T3

51.74

1.36

38.48

2.3

G3

19.53

0.84

21.87

1.64

G3 + C3

41.63

1.12

45.39

2.83

Codon usage

CAI-1d

0.76

0.01

0.71

0.04

CAI-2e

0.65

0.02

0.59

0.04

Ecaif

0.8

0.01

0.79

0.01

  1. All the values are expressed as percentage
  2. Significant differences are indicated in italics
  3. SD standard deviation
  4. a,b,cThe numbers 1, 2 and 3 indicates the first, second and third position of a codon respectively
  5. dCodon adaptation index calculated using CAIcal server
  6. eCodon adaptation index calculated using GenScript Rare Codon Analysis Tool
  7. fExpected codon adaptation index calculated using CAIcal server
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Microbial Cell Factories

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