Table 1 List of the forward (F) and reverse (R) primers used for the amplification of the different DNA coding sequences
From: Effects of mRNA secondary structure on the expression of HEV ORF2 proteins in Escherichia coli
Primers | Sequences |
|---|---|
146-NCOI-F | TTT CCATGG CTCCTTCTCGCCCTT |
Mutated 146-NCOI-F | CCC CCATGG ATGATTCTCGCCCTT |
146-XHOI-R | TTT CTCGAG TGCGAGGACACCGAC |
NcoI-E2s-NCOI-F | TTT CCATGG TTTCCCCTGCTCCTTCTCG |
Mutated NcoI-E2s-NCOI-F | CCC CCATGG TTTCCCCTGATGATTCTC |
NcoI-E2s-XHOI-R | TTT CTCGAG TGCGAGGACACCGAC |
216-NCOI-F | CCC CCATGG ATAAGGGGATAGCTAT |
216-XHOI-R | CCC CTCGAG GCCCTGAAGGCCGAGC |
222-NCOI-F | GGG CCATGG TTATCCAGGACTATGA |
222-XHOI-R | CCC CTCGAG ATACTCCCGGGTTTTACCCC |
231-NCOI-F | CCC CCATGG ATATTGATCTTGGTGAGTCC |
231-XHOI-R | CCC CTCGAG ATACTCCCGGGTTTTACC |
HBsAg-1-NCOI-F | CCC CCATGG AGAGCACAACATCAGGA |
Mutated HBsAg-1-NCOI-F | CCC CCATGG AGAGCACAACATCAGATTTCC |
HBsAg-1-XHOI-R | CCC CTCGAG AATGTATACCCAAAG |
HBsAg-2-NCOI-F | CCC CCATGG CTCTGCTGCTATGCCTCATC |
Mutated HBsAg-2-NCOI-F | TTT CCATGG CTCTGCTTCTGTGCCTC |
HBsAg-2-XHOI-R | CCC CTCGAG AATGTATACCCAAAG |
HBsAg-3-NCOI-F | CCC CCATGG AGAGCACAACATCAGGATTC |
HBsAg-3-XHOI-R | CCC CTCGAG CAGACTTGGCCCCCAATACC |