Fig. 1
From: Effects of mRNA secondary structure on the expression of HEV ORF2 proteins in Escherichia coli
Analysis of protein expression by SDS-PAGE and Western blot. a A 15% SDS-PAGE presenting the expression under IPTG induction. It shows the overexpression of p216, p222 and p231 at expected sizes of 24.7, 25.5 and 26.5 kDa respectively; while no extra-bands are visible for p146 (16.7 kDa), NcoI-E2s (16.9 kDa), HBsAg-1 (26.5 kDa), HBsAg-2 (17.1 kDa) and HBsAg-3 (23.9 kDa), compared to the control. M molecular weight marker, C(−) negative control, before IPTG induction. b Western blot analysis using anti-HEV 4C5 monoclonal antibody for HEV proteins and anti-HBsAg monoclonal antibody for HBV proteins. M molecular weight marker, C(+) positive control