Profiling of Saccharomyces cerevisiae transcription factors for engineering the resistance of yeast to lignocellulose-derived inhibitors in biomass conversion

Table 1 Cell growth of STB5 and YAP1 deletion mutants and transformants in the microtiter plate assay

Strains, mutants and transformants^a,b	Cell growth (OD₆₂₀)
	Coniferyl aldehyde (1.2 mM)		Furfural (17.0 mM)		HMF (24.0 mM)		BPL		SPL
	30 h	156 h	30 h	156 h	30 h	156 h	30 h	156 h	30 h	156 h
BY4741	1.06 ± 0.02	1.24 ± 0.02	1.12 ± 0.02	1.14 ± 0.02	0.82 ± 0.02	1.04 ± 0.02	0.56 ± 0.02	1.07 ± 0.02	0.60 ± 0.01	1.10 ± 0.01
BY4741-PB	0.37 ± 0.15	1.41 ± 0.02	0.73 ± 0.05	1.10 ± 0.02	0.54 ± 0.04	0.96 ± 0.05	0.26 ± 0.09	1.18 ± 0.03	0.61 ± 0.06	1.33 ± 0.01
M-Y	0.08 ± 0.01	0.04 ± 0.01	0.79 ± 0.02	1.11 ± 0.01	0.59 ± 0.02	0.90 ± 0.01	0.11 ± 0.01	1.02 ± 0.02	0.12 ± 0.01	1.37 ± 0.03
M-S	0.06 ± 0.01	0.68 ± 0.12	0.06 ± 0.01	1.10 ± 0.05	0.09 ± 0.01	0.16 ± 0.02	0.06 ± 0.01	0.75 ± 0.05	0.08 ± 0.01	1.01 ± 0.03
SIY	0.08 ± 0.01	0.05 ± 0.01	0.66 ± 0.02	1.13 ± 0.02	0.50 ± 0.02	0.86 ± 0.02	0.10 ± 0.01	0.96 ± 0.08	0.12 ± 0.01	1.29 ± 0.02
YIS	0.08 ± 0.01	1.14 ± 0.03	0.08 ± 0.01	1.02 ± 0.01	0.18 ± 0.01	0.67 ± 0.02	0.10 ± 0.02	0.81 ± 0.01	0.20 ± 0.01	0.82 ± 0.04
T-S	0.80 ± 0.13	1.28 ± 0.05	0.96 ± 0.04	1.16 ± 0.01	0.70 ± 0.05	1.01 ± 0.02	0.71 ± 0.04	1.00 ± 0.04	0.76 ± 0.04	1.16 ± 0.02
T-Y	0.92 ± 0.07	1.13 ± 0.06	0.67 ± 0.06	1.05 ± 0.02	0.93 ± 0.04	1.12 ± 0.07	0.73 ± 0.05	0.89 ± 0.03	0.82 ± 0.02	1.22 ± 0.04

^aBY4741, BY4741 host strain; BY4741-PB, BY4741 control transformant with plasmid backbone (PB) without insert; M-Y, deletion mutant of YAP1; M-S, deletion mutant of STB5; SIY, STB5 overexpressed in deletion mutant of YAP1; YIS, YAP1 overexpressed in deletion mutant of STB5; T-S, BY4741 transformant of STB5; T-Y, BY4741 transformant of YAP1
^bThe values were corrected as described in “Methods” section

ISSN: 1475-2859