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Table 2 Whole-cell conversions of fatty acids (1 mM) and MEDAs (5 mM) into ethyl esters and di-esters

From: Combination of ester biosynthesis and ω-oxidation for production of mono-ethyl dicarboxylic acids and di-ethyl esters in a whole-cell biocatalytic setup with Escherichia coli

 

E. coli pE

E. coli palkKL

Ethyl ester/di-ethyl ester concn (mM)

Ethyl ester/di-ethyl ester concn

Octanoic acid

0.72 ± 0.03

ND

Nonanoic acid

0.82 ± 0.12

ND

Mono-ethyl adipate (C6)

0.17 ± 0.00

< 0.01 mM

Mono-ethyl pimelate (C7)

0.12 ± 0.00

0.03 ± 0.00 mM

Mono-ethyl suberate (C8)

0.19 ± 0.02

0.02 ± 0.00 mM

Mono-ethyl azelate (C9)

0.05 ± 0.00

− 0.02 ± 0.00 mM

Mono-ethyl sebacate (C10)

0.06 ± 0.01

− 0.01 ± 0.00 mM

Mono-methyl azelate (C9)

0.50 ± 0.03a

NT

  1. Incubation was carried out with 1.0 gcdw/L, and lasted for 2 h with fatty acids, 3 h for mono-ethyl dicarboxylic acids
  2. ND not detected, NT not tested
  3. aDetected product was methyl-ethyl azelate