Table 1 Plasmids used in this study
Plasmid | Description | Reference |
|---|---|---|
pUC57-alkKcodopt | Codon optimized alkK in pUC57 | This study |
pUC57-atfAcodopt | Codon optimized atfA in pUC57 | This study |
pGEc47 | Contains genes necessary for growth on alkanes (alkBFGHJKL and alkST) in the broad-host-range vector pLAFR1 | [16] |
pET-Duet-alkK | Codon optimized alkK under control of T7 promoter in MCSI of pET-Duet | This study |
pCOM10-alkL | Contains alkL in pCOM10, a broad host range alkane responsive vector | [17] |
pBGTHJKL-atfA | Complete alk-operon; codon optimized atfA under control of separate PalkB | This study |
pBGTHJKL-eeb1 | Complete alk-operon; eeb1 under control of separate PalkB | This study |
pE | alkK and alkL under control of PalkB; codon optimized atfA under control of separate PalkB in pCOM10 | This study |
palkKL | alkK and alkL under control of PalkB in pCOM10 | This study |
pSTL | alkTL in pCOM10 | [5] |
pBGTHJL | alkBGTHJL genes in pCOM10 | [6] |
pE-II | pE with pBR322 ori, AmpR | This study |