CRISPR interference-guided multiplex repression of endogenous competing pathway genes for redirecting metabolic flux in Escherichia coli

Table 2 Effect of CRISPRi-mediated multiplex repression on n-butanol production

CRISPRi repression
Analysis	Quadruple repression	No repression
Metabolite concentrations [g/L]
Butanol	1.06	0.33
Acetate	1.03	2.14
Ethanol	0.0	0.0
Lactate	0.0	0.18
Succinate	1.20	4.62
Glycerol^a	8.21	0.0
Cell growth [OD₆₀₀]	3.96	5.48

ISSN: 1475-2859