Microbial Cell Factories

Table 1 Comparison of the effect of multiplex CRISPRi on byproduct production in E. coli

From: CRISPR interference-guided multiplex repression of endogenous competing pathway genes for redirecting metabolic flux in Escherichia coli

Repression genes					Product concentrations (g/L)				Product change (%)^a
Number	pta	frdA	ldhA	adhE	Acetate	Succinate	Lactate	Ethanol	Acetate	Succinate	Lactate	Ethanol
None					2.78	0.82	7.10	0.68	0	0	0	0
Single	∇				1.92	0.91	7.18	0.27	− 31	+ 10	+ 1	− 59
		∇			2.59	0.21	7.10	0.62	− 7	− 75	0	− 8
			∇		2.97	1.08	1.15	1.33	+ 7	+ 31	− 84	+ 97
				∇	2.58	0.74	6.90	0.03	− 7	− 11	− 3	− 96
Double	∇	∇			2.24	0.49	7.83	0.62	− 20	− 41	+ 10	− 8
	∇		∇		3.30	1.33	3.51	1.07	+ 18	+ 61	− 51	+ 59
	∇			∇	2.09	0.80	7.19	0.14	− 25	− 2	+ 1	− 80
		∇	∇		2.91	0.37	1.76	1.46	+ 5	− 55	− 75	+ 116
		∇		∇	2.66	0.36	7.42	0.08	− 4	− 57	+ 5	− 88
			∇	∇	3.42	0.80	1.39	0.21	+ 23	− 2	− 80	− 70
Triple	∇	∇	∇		3.58	0.73	4.37	1.43	+ 29	− 12	− 38	+ 113
	∇	∇		∇	2.13	0.52	7.67	0.14	− 24	− 37	+ 8	− 79
	∇		∇	∇	3.25	0.96	3.96	0.57	+ 17	+ 17	− 44	− 16
		∇	∇	∇	3.72	0.65	1.56	0.59	+ 34	− 21	− 78	− 13
Quadruple	∇	∇	∇	∇	3.14	0.71	4.57	0.58	+ 13	− 14	− 36	− 14

^aThe percentage reduction of target byproduct was calculated as \(\left[ {\frac{{{\text{iProduct}}\;\left[ {{{\text{g}} \mathord{\left/ {\vphantom {{\text{g}} {\text{L}}}} \right. \kern-0pt} {\text{L}}}} \right]}}{{{\text{Product}}\;\left[ {{{\text{g}} \mathord{\left/ {\vphantom {{\text{g}} {\text{L}}}} \right. \kern-0pt} {\text{L}}}} \right]}}\; \times \;100} \right] - 100\) where iProduct [g/L] and Product [g/L] are concentration of each target byproduct produced in the presence and absence of CRISPRi control, respectively

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