Fig. 5
From: The expression and construction of engineering Escherichia coli producing humanized AluY RNAs
The estimation of yield of pure AluY RNAs. a The results of northern detection. RNAs were extracted from pET-AluY × 14 DE3 bacteria induced by IPTG and digested with DNase I. 5 μg RNAs digested with DNase I were loaded in lane 1, 0.2 μg Alu reference was loaded in lane 2; b IOD values of each lane of the northern blotting results (means of three independent experiments)