Fig. 2
From: The expression and construction of engineering Escherichia coli producing humanized AluY RNAs
Effect of copy number of AluYs on AluY RNA production. a The results of northern detection. 1, 2, 4, 8 or 14 copies of AluY elements were inserted into the pET plasmid to construct pET-AluY plasmids that were transformed into DE3 bacteria that were induced with IPTG (final concentration 0.2 mg/ml) at 37 °C. The northern blot results showed that AluY RNA production amount increased with the increase of AluY copy number; b dyeing result of methylene blue; c IOD values of each lane of the northern blotting results (means of three independent experiments. “*”, the AluY RNAs yield of pET-AluY × 14 plasmid was significantly higher than that of other plasmids (p < 0.01)