Fig. 1
From: The expression and construction of engineering Escherichia coli producing humanized AluY RNAs
The effects of IPTG induction on the bacterial growth and yield of AluY RNAs producing from pET-AluY × 8 DE3 bacteria. a Effects of IPTG induction on the bacterial growth; b effects of IPTG induction time on the yield of AluY RNAs. The top panel shows the results of northern blotting after different time induced by IPTG; the bottom panel shows the loading amount of RNAs in each lane dyed by methylene blue; c IOD values of each lane of the northern blotting results (means of three independent experiments) after different time induced by IPTG. “*”, the yield of AluY RNAs when IPTG induction for 2–6 h was significantly higher than that of other induction times (p < 0.01). d Effects of IPTG concentrations on yield of AluY RNAs. The top panel shows the northern detection results after 4 h induction by different concentrations of IPTG; the bottom panel shows the loading amount of RNAs in each lane dyed by methylene blue