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Table 3 Quantification and function assessment of purified model proteins

From: Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions

Target protein

µg per gram wet beads

µg per gram wet cell mass

Cleavage ratio (%)

Function assessment

GFP

104.9

11.6

6.6

Fluorescence as measured at excitation 395 nm/emission 509 nm

Rv1626

136.7

14.5

6.5

Antigenicity in immunoblot/ELISA assay

ZZ

95.7

14.6

7.6

IgG binding capability in ELISA assay

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Microbial Cell Factories

ISSN: 1475-2859

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