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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions

Fig. 6

SDS-PAGE (15% Bis–Tris Gel/MOPS buffer) analysis of the resulting soluble fractions upon a 16 h incubation as well as the PHA beads pre- and post-cleavage. Lane 1, molecular weight marker; lane 2, TNFα in the soluble fraction, corresponding to the lowest bottom band (~ 18.6 kDa); lane 3, G-CSF in the soluble fraction, corresponding to the lowest bottom band (~ 19.9 kDa); lane 4, IFNα2b in the soluble fraction, corresponding to the lowest bottom band (~ 20.5 kDa); lane 5, PHA synthase-intein-TNFα beads pre cleavage; lane 6, PHA synthase-intein-TNFα beads post cleavage; lane 7; PHA synthase-intein-G-CSF beads pre cleavage; lane 8, PHA synthase-intein-G-CSF beads post cleavage; lane 9, PHA synthase-intein-IFNα2b beads pre cleavage; lane 10, PHA synthase-intein-IFNα2b beads post cleavage. Please note that in lane 2–4 there are one to three co-purified carrying-over proteins which are respectively numbered and indicated with arrows on the left side

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Microbial Cell Factories

ISSN: 1475-2859

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