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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions

Fig. 4

Specific recognition of the eluted Rv1626 antigen by IgG of mice immunized with beads displaying Rv1626. a SDS-PAGE; b immunoblot. Lane 1, molecular weight marker; lane 2, PHA synthase-intein-Rv1626 beads pre-cleavage; lane 3, PHA synthase-intein-GFP beads pre-cleavage as negative control; lane 4, PHA synthase-intein-Rv1626 beads post cleavage; lane 5, PHA synthase-intein-GFP beads post cleavage as negative control; lane 6, Rv1626 eluted (~ 24 kDa); lane 7, GFP eluted as negative control (~ 28 kDa)

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