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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions

Fig. 3

Time course GFP cleavage off PHA synthase-intein-GFP beads as shown by SDS-PAGE (a) and band ratio/intensity change graph of concerned proteins (b). Lane 1, molecular weight marker; lane 2, beads pre cleavage; lane 3, beads after 16 h incubation at pH 8.6 as control; lane 4, beads after 4 h incubation at pH 6; lane 5, beads after 8 h incubation at pH 6; lane 6, beads after 16 h incubation at pH 6; lane 7, supernatant after 16 h incubation at pH 8.6 as control; lane 8, supernatant after 4 h incubation at pH 6; lane 9, supernatant after 8 h incubation at pH 6; lane 10, supernatant after 16 h incubation at pH 6

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Microbial Cell Factories

ISSN: 1475-2859

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