Skip to main content
Fig. 2 | Microbial Cell Factories

Fig. 2

From: Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions

Fig. 2

SDS-PAGE (10% Bis–Tris Gel/MOPS buffer) of the PHA beads pre- and post-cleavage, as well as the resulting soluble fractions upon a 16-h-incubation. Lane 1, molecular weight marker; lane 2, PHA synthase-intein-GFP beads pre cleavage; lane 3, PHA synthase-intein-GFP beads post cleavage; lane 4, GFP in the soluble fraction (~ 28 kDa); lane 5, PHA synthase-intein-Rv1626 beads pre cleavage; lane 6, PHA synthase-intein-Rv1626 beads post cleavage; lane 7 Rv1626 in the soluble fraction (~ 24 kDa); lane 8, PHA synthase-intein-ZZ beads pre cleavage; lane 9 PHA synthase-intein-ZZ beads post cleavage; lane 10, ZZ in the soluble fraction (~ 18 kDa)

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us