Table 3 Strains and plasmids used in this study
Strain or plasmid | Description | Source or references |
|---|---|---|
Strains | ||
 M. extorquens AM1 | Wild-type, pink color, rifamycin-resistant strain | [21] |
 BHBT5 | Adaptive evolved strain of M. extorquens AM1 with butanol tolerance, white color | [33] |
 YHP1 | M. extorquens AM1/pJY80 | This study |
 YHP2 | M. extorquens AM1/pYM02 | This study |
 YHP3 | M. extorquens AM1/pYM03 | This study |
 YHP4 | M. extorquens AM1/pYM04 | This study |
 YHP5 | M. extorquens AM1/pYM05 | This study |
 YHP6 | M. extorquens AM1/pYM06 | This study |
 YHP7 | BHBT5/pYM07 | This study |
 YHP8 | BHBT5/pJY80 | This study |
 YHP9 | BHBT5/pYM09 | This study |
 YHP10 | BHBT5 ΔMETA1_3271 | This study |
 YHP11 | BHBT5 ΔMETA1_4251 | This study |
 YHP12 | YHP10/pYM07 | This study |
 YHP13 | YHP11/pYM07 | This study |
Plasmids | ||
 pCM130 | Low-background xylE promoter-probe vector, TetR | [55] |
 pCM433 | sacB-based allelic exchange vector; ApR, CmR, TetR | [58] |
 pJY80 | pCM80-based, no promoter P mxaF ; TetR | Lab storage |
 pYM02 | pJY80 (P meta1_3616 ::mcr) | This study |
 pYM03 | pJY80 (P coxB ::mcr) | This study |
 pYM04 | pJY80 (P tuf ::mcr) | This study |
 pYM05 | pJY80 (P mxaF ::mcr) | This study |
 pYM06 | pJY80 (P mxaF–mxaF ::mcr) | This study |
 pYM07 | pJY80 (P mxaF ::mcr::P meta1_3616 ::mcr 550–1219 ) | This study |
 pYM09 | pJY80 (P mxaF ::yhxA-ydfG) | This study |
 pYM10 | pCM433 with upstream and downstream fragments of META1_3271 | This study |
 pYM11 | pCM433 with upstream and downstream fragments of META1_4251 | This study |