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Table 2 Proteins of M. extorquens AM1 showing highest homology by BLAST analysis

From: Production of 3-hydroxypropionic acid in engineered Methylobacterium extorquens AM1 and its reassimilation through a reductive route

Description

Protein ID

Gene locus taq

Identity (%)

References

Putative acrylyl-CoA reductase1,2,3

WP_003599507.1

META1_4251

591, 542, 523

[14, 50]

Enoyl-CoA hydratase4

WP_003602251.1

META1_5098

51

[51]

Putative acyl CoA: acetate/3-ketoacid CoA transferase5,6,7

WP_003598031.1

META1_2054

514,445, 446

[51]

Acetyl-CoA synthetase8,9

WP_012752884.1

META1_2531

677, 498

[51]

Putative propionate-CoA ligase (propionyl-CoA synthetase)10

WP_012752709.1

META1_1932

62

[51]

NAD(P)-dependent oxidoreductase11

WP_012753094.1

META1_2846

42

[51]

  1. Homology to 1 Rhodobacter sphaeroides (AcuI GenBank number ABA77575), 2 Ruegeria pomeroyi DSS-3 (SPO_1914 GenBank number AAV95191) and 3 Escherichia coli K-12 substrain MG1655 (YhdH GenBank number NP_417719); homology to 4 Clostridium acetobutylicum ATCC 824 (short-chain-enoyl-CoA hydratase GenBank number NP_349318); homology to 5 Clostridium propionicum (YdiF GenBank number Q9L3F7), 6 Cupriavidus necator (YdiF GenBank number Q0K874), and 7 Escherichia coli (YdiF GenBank number P37766); homology to 8 Escherichia coli (acetyl-CoA synthetase GenBank number P27550) and 9 Salmonella typhimurium (propionate-CoA ligase GenBank number P55912); homology to 10 Ruegeria pomeroyi (propionate-CoA ligase GenBank number Q5LPB4); homology to 11 Pseudomonas denitrificans ATCC 13867(3-hydroxyisobutyrate dehydrogenase GenBank number AGI23197)