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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Production of 3-hydroxypropionic acid in engineered Methylobacterium extorquens AM1 and its reassimilation through a reductive route

Fig. 5

In vitro enzymatic assay detects the acrylyl-CoA production and effect of 3-HP on acyl-CoAs accumulation. a Analysis of acyl-CoAs formed during the reductive conversion of 3-HP by cell extracts of strain YHP8 grown on methanol. Shown are data for the reaction mixture for 10 min before the addition of NADPH and for 10 min after the addition of NADPH; b the extracted ion chromatograms of 3-HP-CoA, acrylyl-CoA and propionyl-CoA analyzed by LC–MS, and a comparison of mass fragments of acrylic acid on GC–MS between the experimental data (red) and NIST data (blue); c comparison of acyl-CoAs accumulation and 3-HP consumption by cell extracts of strain YHP8 with and without the addition of 20 mM 3-HP. YHP8 (BHBT5/pJY80). Data show the mean with error bars indicating standard deviation calculated from three independent biological replicates

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