Fig. 4

¹³C-labeling experiment demonstrates that the reductive route was mainly responsible for 3-HP degradation. Genes yhxA and ydfG encoding β-alanine-pyruvate aminotransferase and 3-hydroxypropanoate dehydrogenase respectively. a Predicted major labeling patterns from ¹³C-labeled β-alanine through the reductive route and oxidative route; b time course of ratios of isotopomers of some intermediates after the switch over from ¹²C-β-alanine to ¹³C-β-alanine in engineered strain YHP9 grown on ¹²C-methanol. M + 0, unlabeled; M + 1, singly labeled; M + 2, doubly labeled; M + 3, triply labeled; M + 4, quadruply labeled. YHP9 [BHBT5/pJY80 (P_mxaF::yhxA-ydfG)]. Data was calculated from three independent biological replicates. Possible genes involved in reassimilation through the reductive route were added accordingly