Fig. 3

Discovers the metabolite change related to 3-HP assimilation. a 3-HP degradation (circle) and growth profile (triangle) in the YHP8 strain with the addition of 200 mg/l 3-HP. YHP8 (BHBT5/pJY80); b comparison of the specific growth rate in the YHP8 strain with the addition of different amounts of 3-HP; c untargeted metabolomics analysis between strain YHP8 with and without the addition of 3-HP. The metabolomic data was processed by PLS-DA. Data was acquired from six independent biological replicates; d the fragmental structures of m/z 840.1436 was predicted by MassFrontier. The mass accuracy between the theoretical mass and experimental mass is shown below the structure; e comparison of intracellular metabolites involved in the serine cycle and EMC pathway between the YHP8 strain with and without the addition of 3-HP. The y axis is the ratio of the intermediates between strain YHP8 with and without the addition of 3-HP. X axis is the concentration (mg/l) of 3-HP in the medium. The average value for the control strain was set to 1. Data show the mean with error bars indicating standard deviation calculated from three independent biological replicates (***P ≤ 0.001; **P ≤ 0.01; *P ≤ 0.05, t test)