Fig. 1

Constructing the heterologous malonyl-CoA pathway in M. extorquens AM1 for 3-HP production. Mcr-N and Mcr-C: N- and C-terminal fragments of malonyl-CoA reductase. Black solid lines represent methylotrophic pathways used for methanol assimilation. Blue dashed lines represent the malonyl-CoA pathway for 3-HP production. Orange dashed lines represent the 3-HP reassimilation pathway, and possible genes involved in reassimilation were added accordingly