Table 2 Strains, plasmids and primers used in this study

Y179

K. marxianus, wild-type

CCTCC (M202031)

280

S. cerevisiae BY4741, MATa, his3-1, leu2-0, met 15-0, ura3

ATCC (201388)

DH5α

E. coli competent cells for genetic manipulation

Invitrogen (18258012)

TrxR

S. cerevisiae over-expressing KmTrxR gene, transformated from 280

This study

425

S. cerevisiae with pRS425 plasmid, transformated from 280

This study

Trx2

S. cerevisiae over-expressing KmTRX2 gene, transformated from 280

This study

Trx3

S. cerevisiae over-expressing KmTRX3 gene, transformated from 280

This study

423

S. cerevisiae with pRS423 plasmid, transformated from 280

This study

Trx2-TrxR

S. cerevisiae over-expressing both KmTRX2 gene and KmTrxR gene, transformated from 280

This study

Trx3-TrxR

S. cerevisiae over-expressing both KmTRX3 gene and KmTrxR gene, transformated from 280

This study

423–425

S. cerevisiae with both pRS423 and pRS425 plasmid, transformated from 280

This study

pRS423

Yeast episomal vector with HIS3 marker

[41]

pRS425

Yeast episomal vector with LEU2 marker

[41]

p425TrxR

KmTrxR gene in pRS425

This study

p423Trx2

KmTRX2 gene in pRS423

This study

p423Trx3

KmTRX3 gene in pRS423

This study

KmTrxR-F

5′-TCCgagctcCCCATGTCAAATGATGAAACG-3′

KmTrxR-R

5′-TCCccgcggACGAGGAACCAACCTTTATT-3′

KmTrx2-F

5′-GAGTCCgagctcCGGTTTGTTTACCAATCGAT-3′

KmTrx2-R

5′ ATAGCGactagtACTTCGACTATGCGTTGGCC-3′

KmTrx3-F

5′-GAGTCCgagctcTGCCAAAACTGAACTTCCAT-3′

KmTrx3-R

5′-ATATATactagtCTTCCTCGGGCAGCTCGGAC-3′