Table 2 Primers used in this study
From: Biosensor-aided high-throughput screening of hyper-producing cells for malonyl-CoA-derived products
Primer name | Sequences (5′-3′) |
|---|---|
GEX-XhoI-fwd | CGGCTCGAGTGACGATCTGCCTCGCGCGT |
GEX-XbaI-rev | TGCTCTAGATCACTGCCCGCTTTCCAGTC |
R6K-NotI-fwd | TAAGCGGCCGCCTAATTCCCATGTCAGCCGT |
R6K-overlap-rev | GACAAGATGTGTATCCACCTTAACTAAGATCCGGCCACGATGCGTCCGGC |
IS-overlap-fwd | ATCGTGGCCGGATCTTAGTTAAGGTGGATACACATCTTGTCATATGATCTACTAGAGCTGATCCTTCAAC |
IS-SacII-rev | AAACCGCGGAGTTAAGGTGGATACACATCTTGTCATATGATCTTAGAAAAACTCATCGAGCA |
lacI-SacII-fwd | AAACCGCGGTATTTTCTCCTTACGCATCT |
amp-NotI-rev | TAGGCGGCCGCTTACCAATGCTTAATCAGTG |
lacZ-F | TTTAAGAAGGAGATATACATATGACCATGATTACGGATTC |
lacZ-R | TTATTTTTGACACCAGACCA |
PBAD-F | CCATAAGATTAGCGGATCCT |
PBAD-R | GAATCCGTAATCATGGTCATATGTATATCTCCTTCTTAAA |
pAH156-PBAD-F | CTCTAGATAAGGAGGAAAAACTCGAGCCATAAGATTAGCGG |
pAH156-lacZ-R | CTCGGTACCCGGGGATCCGCTCTCGAGGTCGACGGTATCG |
lacZ-pAH156-F | CGATACCGTCGACCTCGAGAGCGGATCCCCGGGTACCGAG |
PBAD-pAH156-R | CCGCTAATCTTATGGCTCGAGTTTTTCCTCCTTATCTAGAG |
kan-sp1 | TATCAGGACATAGCGTTGGCTACCCG |
kan-sp2 | CGGCGAATGGGCTGACCGCTTC |
kan-sp3 | GTGCTTTACGGTATCGCCGCTC |
sp-seq | CATCGCCTTCTATCGCCTTCTT |
AD1 | NGTCGASWGANAWGAA |
AD2 | TGWGNAGSANCASAGA |
AD3 | AGWGNAGWANCAWAGG |
AD4 | STTGNTASTNCTNTGC |