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Table 1 Plasmids and strains used in this study

From: Biosensor-aided high-throughput screening of hyper-producing cells for malonyl-CoA-derived products

Strains and plasmids

Description

Source

Plasmids

 pS1

Genes encoding AraC-TAL and 2-PS variant S1 under the control of promoter P tac . Ampr

This study

 pTn10

Transposon Tn10, Kanr

This study

 pPhlD

Gene phlD under the control of PBAD, Kanr

This study

Strains

 BW-WT

BW25113 (ΔaraC), with promoter PBAD controlled lacZ integrated

This study

 ΔrcsA

BW25113 (ΔrcsA)

This study

 ΔfhuA

BW25113 (ΔfhuA)

This study

 ΔcsgA

BW25113 (ΔcsgA)

This study

 ΔtonB

BW25113 (ΔtonB)

This study