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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Flow cytometric quantification, sorting and sequencing of methanogenic archaea based on F420 autofluorescence

Fig. 6

Community analysis of six digesters ah. Column 1: Flow cytometric measurement of the unstained samples with the respective autofluorescent subcommunities F420+. Column 2: The total SYBR Green I stained digester communities. Column 3: SYBR Green stained subcommunity F420+. Column 4: the total DAPI stained digester communities. A mcrA targeted T-RFLP analysis of methanogenic archaea in the fresh samples is shown for each digester. Unidentified T-RFs are indicated in grey. The digesters were fed with a disintegrated straw, b whole plant rye silage, c corn silage, d, e chicken manure, f common duckweed, g Elodea nuttallii and h synthetic organic acids. 1,000,000 total events were recorded for unstained samples ag while 200,000 events were recorded for unstained sample h; 100,000 cell events were recorded in the SYBR Green I stained samples; 200,000 cell events were recorded in the DAPI stained samples. The black arrow marks the control beads (details in “Methods”)

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