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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Flow cytometric quantification, sorting and sequencing of methanogenic archaea based on F420 autofluorescence

Fig. 4

Flow cytometric analysis of a methanogenic enrichment culture (MEC) with sort gates in black (a unstained, b–e stained). a FSC vs. F420+. Subcommunities with high autofluorescent (MEC F420+) and low autofluorescent properties (MEC F420low) can be detected. b 3D visualization of FSC vs. F420+ vs. SYBR Green I. c FSC vs. SYBR Green I plot of the total microbial community. d F420+ vs. SYBR Green I plot used for discrimination of high (MEC F420 + S1 and MEC F420 + S2) as well as low and non-autofluorescent subcommunities (MEC F420− and MEC MF420low. e Position of subcommunities MEC F420 + S1 and MEC F420 + S2 in a FSC vs. SYBR Green I plot. The arrow marks the control beads

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Microbial Cell Factories

ISSN: 1475-2859

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