Fig. 3

Comparisons of different strain growth patterns, plate-cultured morphologies, fermentation and gene expression profiles. a Growth curve of S. griseoviridis NRRL 2427 wild-type (WT) strain, ΔsgvT1/T2 mutants and WT strain complemented with sgvT1–T2; b The cultured morphology of wild-type strains, ΔsgvT1/T2 mutants and WT strain complemented with sgvT1–T2 in M-ISP4 medium plate; c HPLC analysis of GV (black diamond) and VG (black up-pointing triangle) in fermentation extract. (I) wild-type strain, (II) ΔsgvT1 mutant, (III) ΔsgvT2 mutant, (IV) ΔsgvT3 mutant; d RT-PCR analysis of sgvT1–T3 and control boundary gene orf (+2) in wild-type strain and ΔsgvD1, ΔsgvQ, ΔsgvR2, ΔsgvR3, ΔsgvA mutants and ΔsgvA::sgvA strain