Opportunities and challenges in the development of Cutaneotrichosporon oleaginosus ATCC 20509 as a new cell factory for custom tailored microbial oils

Bracharz, Felix; Beukhout, Teun; Mehlmer, Norbert; Brück, Thomas

doi:10.1186/s12934-017-0791-9

Table 2 Summary of reported batch and fed-batch fermentations of C. oleaginosus

From: Opportunities and challenges in the development of Cutaneotrichosporon oleaginosus ATCC 20509 as a new cell factory for custom tailored microbial oils

Year	Authors	Mode	Substrate	C:N (g/g)	T (°C)	O₂ (v/v/h)	pH	V (l)	C_L (g/l)	Y_LX (% g/g)	L_A	C_X (g/l)	Y_LS (g/100 g substrate)	Y_LT (g/l/h)	C_t (h)
1978	Moon [63]	Batch	Whey (lactose)	nd	30	15	5.8	10	9.3	nd	G	nd	20	0.1292	72
		Batch	Whey permeate (lactose)	nd	30	15	5.86	10	15.6	58.2	G	26.8	27	0.2167	72
1978	Moon [1]	Batch	Whey permeate (lactose)	nd	30	1	5.2–5.8	14	9.3	20	F	46.5	15	0.1292	72
1983	Evans [22]	Batch	Semi-defined (glucose)	nd	30	1	5.5	1	33.2	33.2	F	10.2	11.9	0.3689	90
		Batch	Semi-defined (sucrose)	nd	30	1	5.5	1	37.4	37.4	F	11.2	14.8	0.4156	90
		Batch	Semi-defined (lactose)	nd	30	1	5.5	1	39.2	39.2	F	12.5	16.5	0.4356	90
		Batch	Semi-defined (xylose)	nd	30	1	5.5	1	48.6	48.6	F	9.9	17.4	0.54	90
		Batch	Semi-defined (EtOH)	nd	30	1	5.5	1	30.1	30.1	F	8.5	10	0.3344	90
1988	Vega [77]	Fed-batch	Banana juice	nd	30	1	5.2	0.5	6.18	59	G	10.32	nd	0.0858	72
		Batch	Banana juice	nd	30	1	5.2	0.5	7.81	73.7	G	10.6	nd	0.1085	72
		Batch	Banana juice	nd	30	1	5.2	0.5	4.14	28.5	G	14.5	nd	0.0575	72
1988	Ykema [39]	Batch	Whey permeate (lactose)	25	30	> 10%	4.8	1	4.18	18	G	23.2	nd	0.155	27
		Batch	Whey permeate (lactose)	40	30	> 10%	4.8	1	8.85	36	G	24.6	nd	0.199	39
		Batch	Whey permeate (lactose)	70	30	> 10%	4.8	1	11.43	58	G	19.7	nd	0.123	93
		Fed-batch	Whey permeate (lactose)	40	30	> 10%	4.8	1	29.75	35	G	85	nd	0.372	70
1995	Hassan [61]	Fed-batch	Full synthetic (glucose) N-lim	32.4	30	> 20%	5.5	1.8	3.83	22.5	F	17	12.75	0.1472	26
		Fed-batch	Full synthetic (glucose) Fe-lim	3.5	30	> 20%	5.5	1.8	1.62	9	F	18	3	0.0623	26
1996	Meesters [31]	Fed-batch	Semi-defined (glycerol)	20	30	1.2	5.5	0.5	29.5	25	F	118	11	0.59	50
1998	Daniel [93]	Batch	Whey permeate (lactose)	ng	30	ng	5.8	1.5	20	58.8	F	34	20	0.1379	145
1995	Hassan [61]	Batch	Prickly-pear juice	50	30	> 20%	5.5	1.8	8.75	46	G	19.02	21	0.25	35
2010	Liang [94] ⁠	Fed-batch	Crude glycerol		28	0.67	5.5	1.6	13.8	44.2	G	31.2	nd	0.0479	288
2011	Zheng [33] ⁠	Fed-batch	Semi defined (volatile fatty acids)	nd	30	1	7	2	37	nd	G	68.8	nd	0.51	72
2011	Yu [49]	Batch	Wheat straw hydrolysate	nd	28	nd	nd	0.05	5.8	33.5	F	17.2	4.7	0.0345	168
		Batch	Wheat straw hydrolysate, detoxified	nd	28	nd	nd	0.05	4.2	27.1	F	15.6	3.4	0.025	168
2011	Chi [52] ⁠	Batch	Wastewater	10.6	25	nd	66	0.05	0.8	11.1	F	7.5	nd	0.0058	144
2012	Cui [53]	Fed-batch	Semi-defined (glucose)	30	30.2	0.6	5.5	1	17.4	52.9	G	32.9	36	0.0604	288
		Fed-batch	Semi-defined (glucose)	30	30.2	0.6	6	1	21.8	49	G	44.5	26	0.0757	288
2012	Christophe [50]	Fed-batch	Full synthetic (glucose) phase 1	nd	30	0.5	6	4	1.67	16	G	10.46	11	0.0697	24
		Fed-batch	Full synthetic (acetate) phase 2	50	30	0.5	6	4	6.89	51	G	13.5	15	0.1377	50
		Batch	Full synthetic (glucose)	nd	30	nd	nd	0.25	3.31	47.3	G	7	15	0.0345	96
		Batch	Full synthetic (acetate)	nd	30	nd	nd	0.25	0.83	27.8	G	3	19	0.0087	96
2013	Gong [95]	Flask	Corn stover hydrolysate	nd	30	nd	nd	0.05	6	nd	G	nd	nd	0.125	48
		Flask, SSF	Corn stover	nd	30	nd	nd	0.05	7.2	43.4	G	16.5	nd	0.15	48
2014	Xu [76] ⁠	Fed-batch	Mannitol + VFAs	nd	30	0.5	5.5	2	1.3	36.1	G	3.6	nd	0.0271	48
2014	Liang [96]	Flask	Sorghum baghasse hydrolysate	nd	26	nd	nd	0.04	10.8	40	G	27	14	0.075	144
2014	Zhang [75] ⁠	Batch	Acid-thermal pre-treated sludge	18.99	28	nd	6.5	0.15	19.6	52.8	G	37.1	nd	0.4083	48
		Batch	Thermal pre-treated sludge	22.26	28	nd	6.5	0.15	20.4	81.0	G	25.2	nd	0.4250	48
		Batch	Alkaline-thermal pre-treated sludge	15.67	28	nd	6.5	0.15	20.1	51.8	G	38.8	nd	0.4786	42
2015	Beligon [72] ⁠	Batch	Acetate	nd	30	0.1	6	5	0.26	15	G	1.71	6	nd	nd
		Batch	Acetate	nd	30	0.1	7	5	0.4	18.0	G	2.23	9	nd	nd
		Fed-batch	Acetate	10	30	0.1	7	5	12.0	15.0	G	80	5.4	0.2600	60
2016	Gong [46]	Flask	Semi defined (glucose, xylose, acetic acid)	72	30	nd	7	0.05	24.5	59.3	G	14.5	17.5	0.2269	108
		Flask	Corn stover hydrolysate	61	30	nd	7	0.05	11.8	60.8	G	6.9	9.6	0.1229	96
2017	Meo [97]	Fed-batch	Semi-defined (glucose)	15	30	0.1	6.5	0.01	20.4	56.7	F	35.8	11	0.2833	72
		Fed-batch	Semi-defined (galactose)	15	30	0.1	6.5	0.01	18.6	61	F	31	13	0.2583	72
		Fed-batch	Semi-defined (mannose)	15	30	0.1	6.5	0.01	8.9	48	F	18	9	0.1236	72
		Fed-batch	Semi-defined (sugar mix)	15	30	0.1	6.5	0.01	21	58	F	35.5	11	0.2917	72
		Fed-batch	Semi-defined (glucose), P-lim	15	30	0.1	6.5	0.01	11	39.3	F	27.9	5	0.1528	72
		Partial recycling	Semi-defined (glucose)	15	30	2	6.5	5	16.2	52	F	31.5	31	0.4263	38
		Partial recycling	Algae hydrolysate	5	30	2	6.5	5	30.6	53	F	58	43	0.3255	94
2017	Park [45]	Flask	VFA broth with glucose	nd	25	nd	5.5	0.25	3.2	32.2	F	1.0	17	0.0889	36

Cultivation modes include batch, fed-batch, flask, simultaneous saccharification and fermentation (SSF) as well as a membrane bioreactor approach. If not stated differently, lipid accumulation is induced via nitrogen limitation. Aeration is given in volume air per volume culture per hour if not stated differently. In some cases, aeration was adjusted by setting oxygen concentration in the culture over a threshold value (> 10, > 20%). Biomass (C_X) and lipid concentration (C_L) are given in g/l, lipid content (Y_LX) is given in % g/g and total lipid productivity (Y_LT) is shown as g/l/h. L_A shows the method of lipid quantification [fatty acid extraction and GC analysis (F) or total gravimetric lipid analysis (G)]. Substrate yield Y_LS is shown in gram lipid per 100 g carbohydrate

Back to article page

ISSN: 1475-2859

Contact us

Submission enquiries: journalsubmissions@springernature.com

Microbial Cell Factories

Contact us