Mutants of Yarrowia lipolytica NCIM 3589 grown on waste cooking oil as a biofactory for biodiesel production

Table 2 Biomass, total lipid yield, yield coefficient, volumetric productivities of biomass and lipid of mutants

Treatment	Mutant	X (g L⁻¹)	L (g L⁻¹)	Y_L/X (g g⁻¹)	Q_X (g L⁻¹ h⁻¹)	Q_L(g L⁻¹ h⁻¹)
No treatment	WT	7.1 ± 0.03^a	3.2 ± 0.03^a	0.45 ± 0.004^a,d	0.074 ± 0.0002^a	0.033 ± 0.0002^a
No treatment	YIB6	10.86 ± 0.01^b	5.97 ± 0.01^b	0.55 ± 0.001^b	0.113 ± 0.0001^b	0.062 ± 0.0001^b
MNNG	YIC4	5.76 ± 0.03^c	2.73 ± 0.03^c	0.47 ± 0.005^c,d	0.059 ± 0.0003^c	0.028 ± 0.0003^c
	YIC5	5.91 ± 0.02^d	2.74 ± 0.02^c	0.46 ± 0.004^d	0.062 ± 0.0002^d	0.028 ± 0.0002^c
	YIC6	6.25 ± 0.03^e	2.23 ± 0.03^d	0.35 ± 0.005^e	0.065 ± 0.0002^e	0.023 ± 0.0003^d
	YIC7	7.1 ± 0.03^a	4.28 ± 0.01^e	0.60 ± 0.004^f	0.073 ± 0.0002^a	0.044 ± 0.0002^e
	YIB2	7.0 ± 0.03^a	3.7 ± 0.02^f	0.53 ± 0.006^g	0.072 ± 0.0006^a	0.038 ± 0.0001^f
	YIB3	5.40 ± 0.03^f	1.92 ± 0.03^g	0.35 ± 0.006^e	0.056 ± 0.0003^f	0.019 ± 0.0003^g
MNNG + cerulenin	YID5	5.73 ± 0.01^c	2.42 ± 0.02^h	0.42 ± 0.006^h	0.059 ± 0.0001^c	0.025 ± 0.0002^h
	YIE1	5.84 ± 0.03^d	3.91 ± 0.01ⁱ	0.67 ± 0.004ⁱ	0.061 ± 0.0003^d	0.041 ± 0.0001ⁱ
	YIE2	4.4 ± 0.03^g	1.57 ± 0.02^j	0.35 ± 0.004^e	0.045 ± 0.0002^g	0.016 ± 0.0002^j

The values indicate the mean ± standard deviation (n = 3). Mean values within a column with different superscript letters (^{a,b,c,d,e,f,g,h,i,j}) differ significantly and were determined statistically using Graph Pad InStat ver 3.10 software (One-way ANOVA, Tukey–Kramer multiple comparison test, p < 0.05). Separate analysis was done for each column. The wild type and mutants were grown on LAM containing 100 g L⁻¹ WCO for 96 h. WT wild type, X biomass, L total lipid yield, Y _L/X lipid/biomass yield coefficient, Q _X volumetric biomass productivity, Q _L volumetric lipid productivity. All yields were determined gravimetrically

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