Strains/plasmids | Relevant features | Source/reference |
---|---|---|
Strains | ||
 S. hygroscopicus var. ascomyceticus FS35 | Mutant, improved ascomycin producer | [7] |
 FS-PIB | FS35 transformed with pIB139 | This study |
 HA-Ccr | FS35 transformed with pIB/ccr | This study |
 HA-Hcd | FS35 transformed with pIB/hcd | This study |
 HA-Hcd-Ccr | FS35 transformed with pIB/hcd/ccr | This study |
 E. coli DH5α | Plasmid construction and general cloning | Takara |
 E. coli ET12567 | pUZ8002, nonmethylating plasmid donor, CmR, KanR | [40] |
Plasmids | ||
 pUC18 | E. coli general cloning vector, AmpR | [41] |
 pIB139 | Integrative E. coli-Streptomyces shuttle plasmid containing oriT, attP, int, aac(3)IV and ermEp* | [42] |
 pIB/ccr | pIB139 based integrative plasmid containing ccr gene, AprR | This study |
 pIB/hcd | pIB139 based integrative plasmid containing hcd gene, AprR | This study |
 pUC/hcd | pUC18 based plasmid containing hcd gene | This study |
 pUC/hcd/ccr | pUC18 based plasmid containing hcd and ccr gene | This study |
 pIB/hcd/ccr | pIB139 based integrative plasmid containing hcd-ccr gene, AprR | This study |