Fig. 5
From: Revisiting Escherichia coli as microbial factory for enhanced production of human serum albumin
Effect of chaperone assistance on the functional production of rHSA. a 12%SDS PAGE showing a change in the level of folding of rHSA in presence of chaperones. Lane UI shows the uninduced cell lysate, Lane SGro (Supernatant), folded rHSA in supernatant in presence of GroEL–ES chaperone system; Lane PGro (Pellet), aggregated protein in the presence of GroEL–ES chaperone system; Lane STF, folded rHSA in supernatant in presence of TF chaperone; Lane PTF, aggregated protein in the presence of TF chaperone. b Graph shows the effect of chaperone assistance on the solubility of rHSA. Normalized solubility takes into consideration the solubility with respect to the total amount of expressed rHSA. It represents the fraction percentage of the soluble expressed rHSA of the total expressed proteins in the cell lysate. The inset graph represents out of the total expressed rHSA what fraction is present in the soluble fraction (it does not take into consideration the amount of rHSA expressed). Error bars represent standard error of the mean (SEM) with mean having 95% confidence limit. c Graph shows the effect of chaperone assistance on the activity of rHSA. One unit of enzyme activity corresponds to one nanomole of pNP produced from pNPA per minute. Enzymatic activity expressed in the units of-nanomoles of pNP released/min/g dry cell weight. Error bars represent standard error of the mean (SEM) with mean having 95% confidence limit