Fig. 3
From: Revisiting Escherichia coli as microbial factory for enhanced production of human serum albumin
Effect of osmolytic assistance on the functional rHSA recovery during cell lysis. a 12% SDS PAGE showing the change in the level of folding of rHSA in the absence (Process condition L) and presence of osmolyte (Process condition M). Lane M, medium range molecular weight marker; Lane UI, Uninduced cell lysate; Lane Sc (supernatant control), folded rHSA in supernatant in absence of osmolyte; Lane Pc (pellet control), aggregated protein in the absence of osmolyte; Lane So, folded rHSA in supernatant in presence of osmolyte; Lane Po, aggregated protein in the presence of osmolyte. b Graph shows the effect of osmolytic assistance during rHSA extraction on rHSA solubility and activity. The control and osmolyte panel represents the absence and presence respectively of osmolytic assistance during cell lysis for extraction of rHSA. The black bar represents the solubility of the rHSA depicted in terms of the percentage of the rHSA obtained in the soluble fraction of the total expressed rHSA. The gray bar represents the activity units obtained from carrying out the activity assay of the rHSA. One unit of enzyme activity corresponds to one nanomole of pNP produced from pNPA per minute. Enzymatic activity expressed in the units of-nanomoles of pNP released/min/g dry cell weight. Error bars represent standard error of the mean (SEM) with mean having 95% confidence limit