Fig. 1

Cloning of HSA. a 1% agarose gel showing amplified PCR product and pET23b vector. Lane M, 1 kb DNA ladder; Lane 1, Uncut pET23b plasmid; Lane 2, a double digested pET23b vector with NheI and XhoI restriction enzymes; Lane 3, PCR amplified a product of HSA gene at an optimized annealing temperature of 58 °C. b 1% agarose gel showing the release of HSA gene after double digestion of constructed pETHSA vector with NheI and XhoI restriction enzymes. Lane M, 1 kb DNA ladder; Lane 1, Undigested pETHSA plasmid; Lane 2, digested pETHSA construct. c 12% SDS PAGE showing expression of rHSA in different strains of E. coli. Lane UI, Uninduced cells; Lane 1, Induced BL21 (DE3) E. coli cells; Lane 2, Induced Rosetta (DE3) E. coli cells; Lane 3, Induced Origami2 (DE3) E. coli cells