Fig. 4

The ethanol production of C. thermocellum with different pyruvate decarboxylase genes and T. saccharolyticum alcohol dehydrogenase. a Two colonies were selected for each candidate pdc gene and evaluated by batch fermentation in MTC-5 medium with 30 g/L cellobiose. Three biological replicates were measured. b Ten colonies with AdhA and ApPdc were cultured in MTC-5 medium with 30 g/L cellobiose. Data for each colony is represented by a single point. All the P values were calculated based on the control strain LL1004 with only AdhA. 10 μg/mL thiamphenicol was added to maintain the plasmid. The box includes the 25th to 75th percentile of data; whiskers represent 1.5 times the interquartile range