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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Soluble polymorphic bank vole prion proteins induced by co-expression of quiescin sulfhydryl oxidase in E. coli and their aggregation behaviors

Fig. 2

Production of rBVPrP-109M in the cytoplasm of E. coli. a Coomassie blue staining of the SDS-PAGE gel showing rBVPrP in the insoluble fraction. Lanes 1, 3, 5, 7, and 9 without QSOX; lanes 2, 4, 6, 8, and 10 with QSOX. Lanes 1 and 2, before induction; lanes 3 and 4, 1 h after induction; lanes 5 and 6, 2 h after induction; lanes 7 and 8, 5 h after induction; lanes 9 and 10, 16 h after induction; lane 11, molecular markers (MM). b Western blotting of rBVPrP in the insoluble fraction over time probed with the anti-His antibody. c Quantification of the protein in the insoluble fraction over time. d Coomassie blue staining of SDS-PAGE gel showing PrP in the soluble fraction over time as in a. e Western blotting of rBVPrP in the soluble fraction over time probed with the anti-His antibody. f Quantification of the expression of PrP in the soluble fraction over time

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