Table 1 Oligonucleotides used
Name | Sequence 5′–3′ | Use |
|---|---|---|
LS-005 | TTTTTTCATATGTCCATCACCGCCAGCGAAG | Forward (Fd) for yefMsl amplification. NdeI sequence is underlined |
LS-007 | TTTTTTAAGCTTCACGCCCGCTCCGCGTCCG | Reverse (Rev) for yefMsl amplification. HindIII sequence is underlined |
LS-008 | TTTTTTCATATGAGGATCACTTTCACGTCCCAC | Fd for yoeBsl amplification. NdeI sequence is underlined |
LS-009 | TTTTTTCTCGAGTCAGTAGTGGTAGCGCGCCTGG | Rev for yoeBsl amplification. XhoI sequence is underlined |
LS-113 | CGACTGATGTCATCAGCGGTGG | Fd for aac(3′)IV (apramycin resistance gene) amplification |
LS-114 | CCAACGTCATCTCGTTCTCCGC | Rev for aac(3′)IV (apramycin resistance gene) amplification |
LS-124 | ATGATTGAACAAGATGGATTGCACG | Fd for aph(3′) (neomycin resistance gene) amplification |
LS-125 | TCAGAAGAACTCGTCAAGAAGGCG | Rev for aph(3′) (neomycin resistance gene) amplification |
MRG-11 | TTTTTTCATATGGCCCGCAGACTCCGCACC | Fd for amy amplification. NdeI sequence is underlined |
MRG-12 | TTTTTTCTCGAGGCCGCGCCAGGTGTCGTTGAG | Rev for amy amplification. XhoI sequence is underlined |
LS-116 | CATATGGCTCAGAATCCCCCGG | Fd for xysA amplification |
LS-117 | CTCGAGCGCGGCGAGCACCG | Rev for xysA amplification |