Fig. 6From: Development of an antibiotic marker-free platform for heterologous protein production in Streptomyces Enzyme production after strains storage and after serial subcultures. a and c Amylase and xylanase production by S. lividans ΔTA-Tox transformed with pRoxAnti-Amy (a) and pRoxAnti-Xyl (c) after mycelia storage (M) and after sporulation (S). 10 µL of the supernatant collected after 6 days was loaded into each track. b and d Percentages of amylase (b) and xylanase (d) activity of the supernatants compared with the original culture (1). e and g Amylase and xylanase production by S. lividans ΔTA-Tox transformed with pRoxAnti-Amy (e) and pRoxAnti-Xyl (g) after three 100-fold serial dilutions every 2 days (P1, P2, and P3) in fresh YES medium supplemented with 3% xylose. 10 µL of supernatants collected after 6 days was loaded into each track. f and h percentage of amylase (f) and xylanase (h) activity of the supernatants. The histogram bars are the mean of three experimentsBack to article page